Abstract
Ultrasonic-microwave assisted extraction (UMAE) of Trametes orientalis polysaccharides was optimized by response surface methodology. Hepatoprotective effects of a purified T. orientalis polysaccharide (TOP-2) were evaluated by alcohol-induced liver injury model mice. The optimal UMAE parameters were indicated as below: ratio of water to raw material 28 mL/g, microwave power 114 W, extraction time 11 min. The polysaccharides yield was 7.52 ± 0.12%, which was well consistent with the predicted value of 7.54%. Pre-treatment with TOP-2 effectively increased the liver index and spleen index in alcohol-treated mice. The elevated serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels of mice after alcohol exposure were inhibited by TOP-2 administration. The liver tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) levels have decreased significantly as a result of alcohol exposure, while pre-treatment with TOP-2 could mitigate these consequences. Furthermore, pre-treatment with TOP-2 could efficiently boost the superoxidase dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities, and observably constrain the malondialdehyde (MDA) level. The findings suggest that TOP-2 might be useful for alleviating the alcohol-induced hepatotoxicity via its antioxidant and anti-inflammatory potential.
Highlights
IntroductionAlcoholic liver disease (ALD) is one of the most prevalent liver diseases throughout the world [1]
Alcoholic liver disease (ALD) is one of the most prevalent liver diseases throughout the world [1].ALD can progress from fatty liver, simple steatosis to more serious hepatopathy, for instance, hepatitis, fibrosis, cirrhosis, and even hepatocellular carcinoma [2]
120 W was initially chosen as the optimal microwave power for Ultrasonic-microwave assisted extraction (UMAE) of T. orientalis polysaccharides
Summary
Alcoholic liver disease (ALD) is one of the most prevalent liver diseases throughout the world [1]. The first objective of our work was to see if TOP-2 possesses any hepatoprotective results provide a basis for exploring TOP-2 as a possible antioxidant to ameliorate alcohol-induced activity against liver damage due toofalcohol exposure mice. We used ultrasound-assisted extraction extract polysaccharides from T. orientalis activity against liver damage due to alcohol exposure in to mice. Extraction using of in high-energy effect of ,ultrasonic-microwave we found that it was assisted hard to control the (UMAE), ultrasonicfully temperature the UAE procedure. Ultrasonic-microwave assisted extraction (UMAE), fully of high-energy effect of from different materials, such as mushrooms and herbal plants [18,19,20,21]. Surface microwave and ultrasonic cavitation, has been effectively applied for extraction Response of polysaccharides methodology is ansuch efficient statistical and method forplants the investigation of complex processes. Of the research was to optimize the UMAE parameters of polysaccharides from T. orientalis using RSM
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