Abstract
This research targeted recovering tannins and flavonoids from spent tea leaves using natural deep eutectic solvents (NADES) combined with ultrasonic-assisted extraction (UAE). NADES were produced by organic acids with choline chloride, polyols, and sugars, then the extraction performance of NADES was investigated. NADES from acetic acid-glycerol (NADES3) expressed the highest extraction efficiency of flavonoids and tannins. The single-factor experiments played a role in investigating the influence of UAE conditions on the extraction efficiency of tannins and flavonoids before the Box-Behnken design model was utilized to optimize the UAE processes. The optimum extraction efficiency of flavonoids and tannins was 20.4 mg RE/g db and 185.9 mg CE/g db, respectively, at 900 W of ultrasonic power, 41 mL/g of liquid-to-solid ratios, 69 °C, 20 min, and 10% of water content in NADES. After that, the biological activity of extracts acquired at optimal conditions was quantified. This research illustrates that NADES-based UAE is a green and sustainable method for efficiently recovering tannins and flavonoids from spent tea leaves.
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