Optimization of triazo Acid Black 210 dye degradation by Providencia sp. SRS82 and elucidation of degradation pathway

Abstract

The current work is aimed to evaluate the degradation of triazo textile dye Acid Black 210 (AB210) by Providencia sp. SRS82 that degrade 100mg/L dye within 90min under optimum conditions and was also found tolerant to as high as 2000ppm of dye AB210. Optimum conditions for decolourization and degradation of AB210 with the isolate were viz. temperature 30°C, pH 8, NaCl concentration 2.5% (w/v) and initial cell load of 8×108 cells/mL under static condition. Induction of intracellular and extracellular lignin peroxidase, intracellular laccase and tyrosinase, azoreductase, and DCIP reductase indicated their contribution in the biodegradation of AB210. The products obtained from Providencia sp. SRS82 degradation was monitored through UV–Vis spectrophotometer and were characterized by FTIR, HPTLC, HPLC, GC/MS and LCMS. The proposed metabolic pathway for the biodegradation of AB210 is elucidated for the first time, which showed production of 4 molecules of benzene, one of naphthalene and 4-aminophenyl-N-(4-amino phenyl) benzene sulphonamide. Microbial toxicity and cytotoxicity studies revealed the comparatively less toxic nature of metabolites generated after degradation of AB210. Providencia sp. SRS82 was found competent to degrade actual effluent and diverse dyes that could be present in textile industry effluent showing usefulness of the organism for possible commercial application.