Optimization of the technology of clonal micro-propagation of cherry varieties and clonal rootstocks at the initial stages of in vitro cultivation

O.V Ostrikova,V.V Shakhov,I.E Fedotova,E.L Kharkhardina,S Knyazev,V Kukhar,O Loretts,M Tsoy,O Panfilova

doi:10.1051/e3sconf/202125404003

Abstract

The article presents the results of studying some components of the in vitro technology of clonal rootstocks and common cherry varieties, which allow to increase the efficiency of its application to the tested cherry varieties and clonal rootstocks. Differences in the viability and intensity of the development of clonal cherry rootstocks and varieties explants depending on the timing of their introduction into the crop, the characteristics of genotypes and the composition of nutrient media were established. The influence of nutrient medium composition, content and ratio of growth regulators and genotype in it on the multiplication coefficient of cherry varieties and clonal rootstocks was revealed. The optimal calendar dates for the introduction of cherry varieties and clonal rootstocks explants into the crop in vitro for the autumn-winter-spring period are established. The optimal composition of the culture medium for the initial stages of clonal micropropagation is established. The reproductive potential of some clonal rootstocks and cherry varieties in crop in vitro was revealed.

Highlights

Common cherry (Р. сerasus L. or C. vulgaris Mill.) is one of the most popular stone fruit crops
It is established that the dates of plant explants introduction into the crop, their genotype and the composition of the culture medium are effectiveness factors of the technology of clonal micro-propagation of cherry clonal rootstocks and varieties at the initial stages of cultivation
The best calendar dates for the autumn-winter-spring period for the introduction of common cherry varieties and clonal rootstocks explants into the crop in vitro are February and March, corresponding to the period when the vegetative buds of annual shoots of the original forms are in the state of induced dormancy

Summary

Introduction

Common cherry (Р. сerasus L. or C. vulgaris Mill.) is one of the most popular stone fruit crops. On the territory of Russia, the industrial culture of common cherry has mainly developed in the middle zone of fruit growing (Central Chernozem, Central, North-Western (western regions), Lower Volga (except for the Astrakhan region), Middle Volga regions). Despite the fact that more than 80 common cherry varieties are included in the State Register for the middle zone of fruit growing, this stone fruit crop has practically lost its industrial significance here and is cultivated to a greater extent in amateur gardening [1,2]. One of the reasons for this situation in the Central region of Russia is the lack of highquality virus-free planting material of this crop.

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Conclusion