Optimization of the Production of an Amylase from a Marine Actinomycetes Nocardiopsis Dassonvillei Strain Kas11

Abstract

Marine habitats harbor many extremophilic microorganisms. Actinomycetes are highly significant due to their abilities to produce secondary metabolites and industrial important enzymes. However majority of the actinomycetes so far are investigated from soil and compost and only limited attention is paid to these microorganisms from different saline habitats. Recent studies have indicated that halophilic actinomycetes are potential candidates for many commercially useful enzymes, suitable in detergent and sugar processing industries and bioremediation. In this report, the study based on the production optimization of an amylase is described. Various physical and chemical parameters affecting the amylase production in submerged fermentation are investigated. The actinobacterial strain Nocardiopsis dassonvillei strain KaS11 was isolated from the sea water of Kachhighadi, near Dwarka Coast. The effect of pH, temperature, incubation time, substrate and NaCl concentrations were assessed and optimized by one variable at a time (OVAT) approach. Three folds increase in the enzyme secretion was attained. The halophilic nature of the enzyme was established as the significant enzyme secretion occurred at 1-2 M of NaCl concentrations. Based on the optimization findings, a complete production media was designed for the amylase production.