Optimization of the Preparation Technology of Resistant Starch of Cowpea Using Response Surface Methodology

Abstract

Response surface methodology was used to optimize the preparation technology of resistant starch (RS) production by raw cowpea bean starch. In the first optimization step, single factor experiments designed was used to evaluate the influence of RS yield. The RS yield were influenced significantly by some factors of preparation RS, including the starch concentration, autoclaving time, pullulanase dosage and enzymolysis temperature. The others in the investigation scope had no significant influence on the RS production. In the last step, four main factors were further optimized using Box-Behnken designs and response surface analysis. The optimized conditions in the process of preparation RS were starch concentration as 29%, autoclaving time as 38min, pullulanase dosage as 4.0PUN/g, enzymolysis temperature as 60°C. In our optimal conditions, rather good RS yield was 23.52±0.15% and repeatability of the preparation process was good which was valuable in further production.