Abstract
Based on the assessment of the trophic needs of basidiomycete Flammulina velutipes and the optimization of the composition of the nutrient medium using design of experimental approach, the yield of the submerged biomass of the fungus was increased to 41 g/L, the duration of the cultivation process was reduced to 5 days. For this purpose, the 24 full factorial design, the Box–Wilson steep ascent method and the construction of the response surface were used. Using the method of scanning electron microscopy, it was shown that the studied F. velutipes strain grew in the form of small spherical pellets with a diameter of 1–2 mm on an optimized medium. The surface of the pellets was loose; the inner part was filled with hyphae tightly adjacent to each other. The center of the pellets had no cavity. F. velutipes pellets were formed by septate hyphae with clamp connections. The micromorphological characteristics of the submerged F. velutipes mycelium ensured a high diffusion of nutrients and oxygen into the pellets and their maximum filling of the volume of the culture medium.
Highlights
The development of a nutrient medium for growing any producer consists of two stages: the choice of nutrition sources and the establishment of their optimal quantitative ratio
Already at the stage of screening nutrition source, it is desirable to obtain a sufficiently high yield of the target product. This is a prerequisite for achieving success at the stage of optimizing the quantitative ratio of nutrient medium ingredients
Stage, composition of the nutrient mecarbon and and nitrogen thethe composition of the nutrient medium dium was optimized using the methods of mathematical planning of the experiment
Summary
Active research on culinary-medicinal basidiomycetes is associated with their ability to synthesize metabolites of various chemical nature and biological effects. Due to its high nutritional value and flavorful taste, the fruit bodies of F. velutipes are grown on a large scale using solid-state cultivation. Fruiting bodies and vegetative mycelium of F. velutipes contain biologically active metabolites: polysaccharides, peptides, and sesquiterpenes. Polysaccharides of F. velutipes possess antioxidant, immunomodulatory, anti-tumor, anti-inflammatory, antiviral, cognitive-enhancing, hepatoprotective and some other properties [1,2,3,4]. Peptides exhibiting immunomodulatory properties were isolated from F. velutipes [5]. Active metabolites of F. velutipes with low molecular weight are mainly represented by sesquiterpenes, which display antibacterial, antifungal, antimalarial, or cytotoxic activity [6]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
