Abstract
Abstract A host-vector system was used for the production of Streptomyces subtilisin inhibitor (SSI). The gene fragment encoding SSI was replaced in the vector with the tyrosinase gene of plasmid pIJ702. It was found that the optimal culture conditions for the SSI production by the former system are almost the same as those for the melanin synthesis by the latter system. This fact suggests a convenient method in that the information on the productivity of an indicator host-vector system with regard to the culture conditions can be applied for the optimization of the production of a different material with a similar host-vector system differing in the gene coding for the different product.
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