Optimization of the method of determination of the residual amounts of florasulam in agricultural crops

Abstract

The improved method for determination of florasulam in green mass, grain and straw of cereal crops was presented in this paper. Optimal conditions were chosen for extraction of the substance and purification of the obtained extracts from co-extractive substances by cartridges for solid-phase extraction of Diapak C. For the analysis of florasulam, isocratic reversed-phase high-performance liquid chromatography with a UV detector was used. Sufficient sensitivity of the detector and optimal chromatographic conditions allowed us to determine florasulam at a concentration of 0.125 ?g/ml. The detection limit in the grain was 0.025 mg/kg, in straw and green mass – 0.05 mg/kg. The percentage of florasulam using the optimized method exceeded 80 %.