Optimization of the Isolation Procedure of Genomic DNA from a Mucus Laden Pulmonate Gastropod, Achatina fulica

Abstract

Genomic DNA of good quality is a prerequisite in molecular biology studies involved in deciphering the information contained in the molecules that are chosen for studying the evolutionary interrelationships among taxa. Genomic DNA should be free of contaminants which may otherwise interfere while performing PCR. This is particularly essential while isolating genomic DNA from gastropods due to the presence of large amounts of mucopolysaccharides in their tissues. In the present study, we have developed a new method for obtaining high quality genomic DNA from a pulmonate snail Achatina fulica, which secretes a large amount of mucus. In this method, we have obtained good quality genomic DNA, compatible for downstream molecular biology applications like PCR and sequencing of 16S rDNA. The yield of the genomic DNA was 2790 µg/ml. This method may be extended for isolating high quality genomic DNA from different gastropods native to Indian sub-continent, which serves as a starting point for studying their phylogenetic affinities.