Optimization of the Green Synthesis of Silver Nanoparticle with Box-Behnken Design: Using Aloe Vera Plant Extract as a Reduction Agent

Abstract

Nowadays, many of plants are used as a reduction agent in biosynthesis of silver nanoparticles. In this study, green synthesis of silver nanoparticles was aimed to optimize with Box-Behnken design. Aloe vera plant extract was utilized as a reduction agent as it is the famous natural product in field of cosmetic and skin health care. The synthesized silver nanoparticles using Aloe vera plant extract solution were optimized by Box-Behnken design due to the influence of different factors such as microwave power, time, AgNO3 concentration, and ratio of volume of Aloe vera plant extract solution to volume of AgNO3 and the percentage yield of particle formation as a response. Quadratic polynomial model was used to carry out mathematical modelling and response surface analysis was performed to determine the independent variable-response relationship. The optimized silver nanoparticles were characterized using instrumental devices like FTIR spectroscopy UV-VIS spectrophotometry. In addition, the optimized silver nanoparticles were tested for antibacterial activity on Gram positive (S. aureus, E. faecalis) and Gram negative ( P. aeruginosa, K. pneumoniae )bacteria. Briefly, the trials interpreted successful synthesis of the silver nanoparticles along with comprehension of the related factors affecting their quality characteristics and remarkably developed antibacterial activity as helpful impact.