OPTIMIZATION OF THE EXTRACTION PROCESS OF HORSE CHESTNUT (AESCULUS HIPPOCASTANUM L.) POLYSACCHARIDES

Abstract

The results of the development of optimal parameters for the extraction process of polysaccharides isolated from the shells of horse chestnut fruits collected on the territory of Tashkent and the Tashkent region of the Republic of Uzbekistan are presented. To identify the optimal indicators affecting the extraction processes, the influence of the following parameters on the yield of polysaccharides was studied: the ratio of raw materials: extractant, the extraction rate, the extraction temperature, the degree of evaporation. It has been established that at ratios of raw materials : extractant of 1 : 30, the highest yield of the final product is observed leads to a significant increase in the yield of the final product. The influence of the temperature regime on the yield of polysaccharides was carried out in the temperature range from 25 to 120 °С. At the same time, under constant conditions of the bath modulus of 1 : 30 and the duration of extraction of 2 hours with varying temperature, the highest yield of polysaccharides is observed at a temperature of 95 °С. The optimal degree of evaporation of the extract, in which no large losses of the final product are observed, is the degree of evaporation by 4 times. To assess the influence of technological parameters on the change in qualitative or quantitative indicators of the quality of PS, a one-way analysis of variance was studied for each experiment. Analysis of variance showed the significance of the Fisher criterion, since the p-value is less than 0.05, therefore, the influence of the studied parameters on the yield of polysaccharides can be considered proven. Keywords: extraction, aqueous fraction, extractant, analysis of variance.