Optimization of the experimental parameters in the determination of rifamycin SV by adsorptive stripping voltammetry

Abstract

A procedure for the determination of rifamycin SV by adsorptive stripping voltammetry (AdSV) has been optimized. Two different voltammetric techniques, namely differential pulse adsorptive stripping voltammetry (DPAdSV) and square wave adsorptive stripping voltammetry (SWAdSV) were used. In order to determine the most sensitive technique, an optimization of the experimental parameters was carried out for every technique using experimental design methodology. A canonical analysis of the response surface was carried out, which was adjusted to determine the optimum and the influence of the experimental factors. When this lay outside the ambit of the experiment, the optimum path method was used for the optimising process. The detection limits obtained, 3.108 × 10 −8 mol dm −3 for DPAdSV and 1.233 × 10 −8 mol dm −3 for SWAdSV, show that SWAdSV is a more sensitive technique for the analysis of rifamycin SV. Finally, this method was used to determine rifamycin SV in real samples.