Abstract
The applicability of the ETS method for natural phytoplankton assemblages was tested with a number of freshwater phytoplankton species: Scenedesmus quadricauda, Chlamydomonas reinhardtii, Oscillatoria agardhii and Stephanodiscus hantzschii. The reproducibility and recovery of the method is very good when membrane filters are used and the concentrated sample is removed from the filter by brush. Three to five minutes appear to be sufficient to release the ETS activity. The following aspects conditions should be taken into account: (1) The processed sample stored for not longer than 30 min on ice; (2) the extinction must not be higher than 0.6, (3) reaction time not longer than 30 min, and (4) no “hard” species must dominate the water sample. The recovery of the size-fractionated water sample, based on the activity of the whole sample, was not significantly different from 100% at the level of P = 0.01.
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