Abstract
A highly sensitive electrochemical biosensor with a signal amplification platform of electrodeposited gold nanoparticle (AuNP) has been developed and characterized. The sizes of the synthesized AuNP were found to be critical for the performance of biosensor in which the sizes were dependent on HAuCl4 and acid concentrations; as well as on scan cycles and scan rates in the gold electro-reduction step. Systematic investigations of the adsorption of proteins with different sizes from aqueous electrolyte solution onto the electrodeposited AuNP surface were performed with a potentiometric method and calibrated by design of experiment (DOE). The resulting amperometric glucose biosensors was demonstrated to have a low detection limit (>50 μM) and a wide linear range after optimization with AuNP electrodeposition.
Highlights
Label free biosensors with potentiometric signal generation and transduction are of great interest because they would significantly decrease the cost and time needed for sample preparation and provide an easy-to-use tool to monitor binding events [1,2,3,4,5,6]
We found that the electrodeposition of the AuNPs within different potential ranges exhibit different changing gold reduction peaks at different potentials
The preparation of of aa label-free label-free highly-sensitive highly-sensitive biosensor the electrodeposition electrodeposition of
Summary
Label free biosensors with potentiometric signal generation and transduction are of great interest because they would significantly decrease the cost and time needed for sample preparation and provide an easy-to-use tool to monitor binding events [1,2,3,4,5,6]. The principle of potentiometry is measuring the change of surface potential (working electrode) against to constant surface potential (reference electrode). Nowadays, potentiometric devices such as field effect transistor (FET) and light-addressable potentiometric sensor (LAPS) are the most trendy and sensitive electrical biosensors [7,8,9,10,11,12,13]. Sakai et al purposed a method of electrochemical deposition which provides an easy and rapid alternative for preparation of nanoparticle platform in a short time with high AuNPs surface coverage [22]. Gold nanoparticles (AuNPs) can be conjugated with biomolecules and retain the biochemical activity of tagged
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