Abstract
Roseoflavin (RoF), and its metabolic precursor 8-dimethylaminoriboflavin (AF), produced by Gram-positive bacteria Streptomyces davawensis and Streptomyces cinnabarinus, reveal a strong antibiotic effect against Staphyloccus aureus, Bacillus subtilis, Bacillus cereus, Micrococcus luteus and other Gram-positive bacteria and could be promising for developing the new effective antibacterial drugs. In this work, we present the optimization of basic molecular tools for S. davawensis including total DNA preparation, PCR amplification of target gene, cell transformation. In addition, the compositions of cultivation media were studied and the optimal cultivation conditions for increased of RoF production have been developed.
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