Abstract
Numerous factors can influence the growth, reproduction, and metabolism of microbes, which influence the yield and production cost. Bacillus sp. EtOH is a bacterial strain with a high yield of ethanol. In this paper, medium of Bacillus sp. EtOH was optimized. Different carbon/nitrogen sources were investigated, and the best carbon source (sucrose) and nitrogen source (ammonium sulfate) were determined to improve ethanol production. In addition, the best initial pH, temperature, and inoculation volume were 7, 37 °C and 1%, respectively. This study will be conducive to the subsequent ethanol fermentation, and improve application level of Bacillus sp. EtOH.
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