Optimization of the conditions for preparation of silyl derivatives of phenylcarboxylic acids in diethyl and methyl tert-butyl ethers

Abstract

In this work, the optimum conditions of derivatization of six phenylcarboxylic acids (sepsis biom-arkers) in diethyl ether and methyl tert-butyl ether were selected. N,O-Bis(trimethylsilyl)trifluoroacetamide (BSTFA) was used as a silylating agent. The following conditions were chosen as optimum in terms of sensitivity of the silylating agent and its amount: 300 µL of ether and 80 µL of BSTFA, at which the derivatization yield of the studied compounds in model solutions was more than 70% in diethyl ether and more than 85% in methyl tert-butyl ether. The developed conditions will make it possible to increase the derivatization yield at various sample preparation procedures (liquid–liquid or solid-phase extraction) compared to the currently used conditions.