Abstract
In this work, an amperometric biosensor for determination of choline concentration in biological liquids was optimized. The enzyme choline oxidase was used as a bioselective material and was immobilized by crosslinking with bovine serum albumin via glutaraldehyde on the surface of amperometric platinum disc electrode. Influence of working buffer parameters (buffer capacity, ionic strength and pH) and functional characteristics of the biosensor were investigated. Dependence of activity of the immobilized enzyme on solution pH was showed. Furthermore, biosensor storage stability during long period at different conditions was investigated. The storage at −18 °C showed better results. It was showed, that application of semipermeable membrane – poly-m-phenylenediamine, remarkably decreased of the biosensor response to the interfering substances. The main analytical characteristics of developed biosensor such as sensitivity, linear range, detection limit, noise, drift and reproducibility of responses were investigated. The results show that developed biosensor can be used for choline determination in real biological liquids.
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