Abstract
Abstract In this study, enantiomerically pure (S)-1-phenylethanol was produced via asymmetric bioreduction of acetophenone. Ispir bean (Phaseolus vulgaris) was used as an alcohol dehydrogenase (ADH) source since whole cells are cheaper than isolated enzymes. Acetone powder methodology was applied for biocatalyst. Glucose was used as a cosubstrate in-order to regenerate cofactor (NADPH). The reactions were carried out in an orbital shaker whose temperature and agitation rate can be controlled. (S)-1-phenylethanol concentration was analyzed by HPLC using a Chiralcel OB column. Effects of the reaction time, substrate concentration, cosubstrate concentration and biocatalyst concentration on the (S)-1-phenylethanol production were investigated using Response Surface Methodology (RSM). 36 h bioreduction time, 6 mM acetophenone concentration, 25.15 mM glucose concentration, and 175 mg/mL biocatalyst concentration were determined as optimum values. In these conditions, 2.4 mM (S)-1-phenylethanol was obtained in phosphate buffer (pH=7.0) at 30°C with >99% enantiomeric excess.
Highlights
Let F denoteIspir a field andbean let V denote of (S)-1-phenylethanol asa vector space over F with finite po a pair A, A of diagonalizable F-linear maps on V, each of which acts on an eigenb irreducible tridiagonal fashion
Glucose was used as a cosubstrate in-order t reaction time, h to regenerate cofactor (NADPH)
They performed the reduction reaction presence of 2-hexanol and a small amount of a coenzyme, NAD+ and as a result the enantiomeric excess increased from 39% to 99%. They showed that acetone powder of G. candidum reduced acetophenone with 89% yield and 99% enantiomeric excess
Summary
Let F denoteIspir a field andbean let V denote of (S)-1-phenylethanol asa vector space over F with finite po a pair A, A of diagonalizable F-linear maps on V, each of which acts on an eigenb irreducible tridiagonal fashion. Such a pair is called a Leonard pair. Glucose was used as a cosubstrate in-order t reaction time, h to regenerate cofactor (NADPH). HPLC high pressure liquid chromotography carried out in an orbital shaker whose temperature and RSM response surface methodolgy. V denote a vector space over F with finite positive agitation rate can be controlled. (S)-1-phenylethanol pair
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