Abstract
Sugary cassava or mandiocaba is a cassava variety of potential use for bioethanol production. In this study, laboratory-scale fermentations were carried out in a bioreactor with a working volume of 1L, using the yeast strain LNF CAT-1. A central composite design (CCD) was applied to determine the extent to which pH, temperature, and yeast concentration influence ethanol production with the aim of improving the fermentation process. The individual effects and the interaction of these factors were analyzed using a surface response method. Physicochemical properties of the material were also investigated and the analysis of root characterization showed high moisture content (~91%) and a low amount of starch (~4.0%), ash values close to 1.0%, total fibers 0.4%, proteins 0.15%, and lipids 0.1%. The results obtained from the wort presented a low acidity (~0.2%), pH close to neutrality (~6.5%), total soluble solids values of ~5.8%, glucose content ~2.3%, fructose ~1.0%, and sucrose ~1.2%. The second-order polynomial regression model determined that the maximum ethanol production of 2.8% (v/v) would be obtained when the optimum pH, temperature, and yeast concentration were ~5.0, 32-36 ºC, and ~10-14 g L-1, respectively.
Highlights
The development of sustainable energy resources and the reduction of greenhouse gases from fossil fuels have become essential topics of interest worldwide (Pradhan, Mahajani, & Arora, 2018)
The present study evaluated the physicochemical properties of sugary cassava wort and root, as well as evaluated the effect of factors such as temperature, pH, and yeast concentration on alcoholic fermentation for ethanol production
This study illustrates the huge potential sugary cassava has for ethanol production
Summary
The development of sustainable energy resources and the reduction of greenhouse gases from fossil fuels have become essential topics of interest worldwide (Pradhan, Mahajani, & Arora, 2018). It is clear that the replacement of current fossil energy will require the development of new strategies to reduce our global energy consumption and the development of a panel of renewable energy sources (Carneiro et al, 2017). In this scenario, sustainable biofuel production is a valuable tool to curb climate change (Creutzig et al, 2015). As an example of biofuel, bioethanol has received increasing attention due to its excellent properties, mature production technology, and widely available raw material. In addition to corn in the United States and China, sugarcane in Brazil and wheat in some European countries, cassava has been applied to produce bioethanol in many countries, especially tropical countries in Africa, Asia, and Latin America (Kristensen et al, 2014; Zhang et al, 2016)
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