Abstract
Numerous methods have beensuccessfully used to evaluate mammalian spermatogonial biologyHowever, the conventional light microscopy assays present a challenge in precisely identifying spermatogonial phenotypes, which can result in discrepancies between molecular and morphological findings. Such precise association could lead to a more robust interpretation of spermatogonial activityin steady-state spermatogenesis, which may facilitate the translation from basic researchto clinical applications. In this chapter, we present two histological processing methods that enable a comprehensive analysis of spermatogonial morphology and function, involving fixation of mammaliantesticular tissue in glutaraldehyde and embedding in plastic resin. These techniques have proven to be effective in light microscopy studies.
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