Optimization of sugarcane juice as a culture medium for scale up of Lactobacillus plantarum TISTR 2083 production

Abstract

Lactobacillus plantarum TISTR 2083 is one of the industrially relevant lactic acid bacteria vastly used as a probiotic. The culture of L. plantarum TISTR 2083 in sugarcane juice was studied to optimize the culture medium and growth conditions for the highest viable cell production at a low cost. The effect of sugar content (5 - 20°Bx), amount of seed culture (1 - 10%), different nitrogen sources (ammonium sulfate, diammonium phosphate, yeast extract and peptone) with different concentrations (0 – 1.5%), and different agitation speeds (0 – 150 rpm) were investigated in the sugarcane juice growth media for higher cell growth of L. plantarum TISTR 2083. The concentration of sugarcane juice was optimized with 5, 10, 15, and 20°Bx. The highest cell viability was found in the 20°Bx sugarcane juice as 8.90-9.24 log CFU/mL. Moreover, seedlings with a 5% (v/v) seed culture maximized the viable cell (9.07 log CFU/mL) than 1, 3, 7, and 10% seed cultures, respectively. Among different nitrogen sources, high cell viability was obtained using 1.5% yeast extract (8.76-9.11 log CFU/mL). With all other optimized parameters, the agitation speed of 100 rpm resulted in a higher viable cell count. Optimized sugarcane juice can be used as a low-cost medium and a simple bottle fermenter for the scale-up of L. plantarum.