Abstract
In the present study,SRAP-PCR reaction conditions for Opisthopappus taihangensis( Ling) Shih were optimized. The factors affecting SRAP results of O. taihangensis were investigated by uniform designs of two times. The optimal protocol was accomplished in 10 μL reaction volumes containing 1. 3 μL template DNA,0. 35 μL primers and 5. 3 μL 2 × Taq MasterMix. Under this optimal condition,clear and polymorphic bands from 11 O. taihangensis plants had been obtained using Me1 /em4 primers. The result showed that the reaction condition was suitable for SRAP-PCR reaction system of O. taihangensis.
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