Optimization of serum formula active fraction of moringa leaf extract (Moringa oleifera Lambk) as antibacterial against Staphylococcus aureus

Abstract

This study aims to determine the optimum formula for the serum active fraction of Moringa leaves with a combination of carbopol, NaCMC and propylene glycol as antibacterial. Material and methods the fractionation of the extract were tested on Staphylococcus aureus bacteria using the diffusion method. The ethyl acetate fraction which has antibacterial activity at a concentration of 15% was made into serum preparations with a combination of carbopol, NaCMC and propyleneglycol. Making serum formulas using the simplex lattice design method resulted in 14 serum formulas. Testing of the physical quality of the preparations carried out included organoleptic tests, homogeneity, pH, adhesion, viscosity, spreadability, and antibacterial activity. The results of the study on the ethyl acetate fraction of Moringa leaves with a concentration of 15% had antibacterial activity against S. aureus with an inhibition zone of 14.36 ± 1.1 mm. The TLC test the chemical compounds contained in the ethyl acetate fraction of Moringa leaves were flavonoids, saponins and tannins. The optimum combination proportions for serum preparations obtained variation values, namely carbopol 0.67% and NaCMC 3% and propylengliol 5.32%.