Abstract
The aim of this study was optimization of rosmarinic acid extraction from lemon balm (Melissa officinalis). The optimal conditions for the extraction of rosmarinic acid from lemon balm were determined using response surface methodology (RSM). A center composide design (CCD) was used to investigate the effects of three independent variables, namely solid-liquid radio, solvent composition (%) and extraction temperature (°C). Dependent variable was yield of rosmarinic acid. A second-order polynomial model was used for predicting the response. Optimized conditions for rosmarinic acid were: PEME 1:29 (w/v), temperature 66 °C a % propan-2-ol 34 %. The experimental values agreed with predicted within a 95 % confidence interval. Yield of rosmarinic acid extraction by these optimized conditions was achieved 72.6 mg of rosmarinic acid / g of dry extraction matter.
Highlights
Rosmarinic acid is an ester of caffeic acid and 3,4-dihydroxyphenyllactic acid (PEREIRA et al, 2005; PETERSEN, 1996)
The aim of the present study was to evaluate the effect of changing solvent polarity characterisation using dielectric constant, liquid to solid ratio, temperature and time of extraction on the rosmarinic acid yield in extracts
Extraction efficiency of natural compounds is impacted by multiple parameters such as temperature, time and solvent polarity, among others, and their effects may be either independent or interactive
Summary
Rosmarinic acid is an ester of caffeic acid and 3,4-dihydroxyphenyllactic acid (PEREIRA et al, 2005; PETERSEN, 1996). It has a number of interesting biological activities, e. G. antimicrobial (PAL BAIS et al, 2002), antiallergic (ITO et al, 1998; MATSUNO et al, 2002), antiviral and anti-inflammatory (GEORGIEV et al, 2006; HUANG et al, 2006; KAMATOU et al, 2005). Rosmarinic acid is commonly found in species of the Boraginaceae and Lamiaceae. The plants with rosmarinic acid content were used in traditional medicine. Plant extracts found some application in food and cosmetic industry. The aim of the present study was to evaluate the effect of changing solvent polarity characterisation using dielectric constant, liquid to solid ratio, temperature and time of extraction on the rosmarinic acid yield in extracts
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