Abstract
The purpose of these studies was to optimize RHDV type 1 and 2 (RHDV1 and RHDV2) inactivation modes to use the obtained antigens in inactivated vaccines and diagnosticums. The inactivating effect of aminoethylethylenimine and β-propiolactone was studied in different concentrations in correlation with the exposure time and temperature. The correlation between the inactivating effect of the compound used and the accepted test conditions (concentration, temperature, and exposure time) was studied on a group of rabbits, each of which was injected intramuscularly with 1 cm3 of the inactivated material sample. At the end of the maximum exposure interval, a control sample of the viral material, kept under the same conditions without any inactivant added was similarly tested. Lethality was considered to evaluate the damaging action in the test and control groups: L = m/n, where m is the number of dead animals; n is the total number of rabbits in the group for testing of the inactivated material sample. The postmortem diagnosis was confirmed by testing the rabbit liver tissue homogenate for relative antigens using ELISA. It was found that aminoethylethylenimine and β-propiolactone did not have the same effect on the studied variants of the virus. In order to preserve at maximum the antigenic structures of the virus, the following inactivation modes were considered to be optimal: for RHDV1-aminoethylethylenimine at a concentration of 0.3% at 37 °C, exposure time – 72 hours, or β-propiolactone at a concentration of 0.1–0.3% at 25–37 °С, exposure time – 24–48 hours; for RHDV2 – aminoethylethylenimine at a concentration of 1% at 37 °C, exposure time – 72 hours, or β-propiolactone at a concentration 0.3% at 25 °С, exposure time – 24 hours.
Highlights
The inactivating effect of aminoethylethylenimine and β-propiolactone was studied in different concentrations in correlation with the exposure time and temperature
Lethality was considered to evaluate the damaging action in the test and control groups: L = m/n, where m is the number of dead animals; n is the total number of rabbits in the group for testing of the inactivated material sample
The postmortem diagnosis was confirmed by testing the rabbit liver tissue homogenate for relative antigens using ELISA
Summary
Для исследований использовали 10%-ю тканевую суспензию (вес/объем), полученную из ткани печени кроликов, инфицированных RHDV1 и RHDV2. Перед использованием в эксперименте в размороженный материал вносили хлороформ (2% по объему) и высокомолекулярный полигексаметиленгуанидин гидро хлорид (0,1% по объему), активно перемешивали и цент рифугировали при 500 g в течение 20 мин. Определение гемагглютинирующей активности вируса проводили в соответствии с методикой, изложенной в Руководстве МЭБ по диагностическим тестам и вакцинам для наземных животных [1]. Для инактивации вируссодержащих материалов использовали аминоэтилэтиленимин в виде 15%-го водного раствора (АЭЭИ, ООО НПП «Биохимсервис», Россия) и β-пропиолактон (Acros Organics, США). Подготовленные инактиванты добавляли в вируссодержащий материал до заданных конечных концентраций. Через заданные интервалы времени производили отбор проб для определения полноты инактивации вируса. При тех же условиях содержали образец вируссодержащего материала без добавления инактиванта (контроль).
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