Optimization of Quantum Dots system of CdTe and Transferrin by Capillay Electrophoresis-Laser Inductive Fluorescence and Verification in Cell Labeling

Abstract

Abstract Capillary electrophoresis-laser inductive fluorescence (CE-LIF) was used to characterize the active effect difference of coupling agent N -hydroxysulfo-succinimide (NHS) and the mixture of NHS and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimides hydrochloride (EDC) on quantum dots CdTe, and to optimize the conditions of CdTe and transferring (Trf) coupling. The labeling effect of CdTe-Trf and CdTe-BSA complex as well as bare CdTe in Hela cell was compared. The results demonstrated that the property of CdTe activated by NHS was better than that of the mixture of EDC-NHS. When 12.5, 31.2 and 40.6 μM Trf were used to couple CdTe respectively, the resultant of CdTe with 31.2 μM Trf optimized by CE-LIF showed the best labeling on Hela. The CdTe-Trf resultant labeled Hela rapidly on the surface when they incubated at 4 °C for 20 min, and transferred into Hela when they incubated at 37 °C for 7 h, which manifested the CdTe-Trf resultant has biological function. The CdTe-Trf can recognize and bind the Trf receptor on cell membrane specifically and enter Hela, while CdTe-BSA cannot recognize Hela specifically and bare CdTe cannot label on cell.