Optimization of Process Variables by Response Surface Methodology

Kashipeta Ravinder

doi:10.3126/ijasbt.v3i4.13352

Abstract

In the present study optimisation of the growth medium for the production of Cyclodextrin glucanotransferase (CGTase) was carried out using response surface methodology. Four important parameters namely starch, yeast extract, K2HPO4 and MgSO4 concentrations were selected as the independent variables and the enzyme activity (CGTase activity U/mL) was the dependent response variable. Each of these independent variables was studied at five different levels as per central composite design (CCD) in four variables with a total of 28 experimental runs. The optimal calculated values of tested variables for maximal production of CGTase were found to be comprised of: starch, 2.16 %; yeast extract, 0.6 %; K2HPO4, 0.62 %; MgSO4, 0.04 % with a predicted CGTase activity of 150 U/ml. These predicted optimal parameters were tested in the laboratory and the final CGTase activity obtained was very close to the predicted value at 148.2 U/ml.Int J Appl Sci Biotechnol, Vol 3(4): 592-598

Highlights

Cyclodextrin glucanotransferase (CGTases; EC 2.4.1.19) is an enzyme which converts starch into the cyclodextrins (CDs)
High CGTase activity was observed in experimental runs with the mid values of the parameters
It was observed from various experimental runs that CGTase production was quite high with higher starch concentration

Summary

Introduction

Cyclodextrin glucanotransferase (CGTases; EC 2.4.1.19) is an enzyme which converts starch into the cyclodextrins (CDs). Based on the number of glucose moieties the CDs are classified as α-, β-, and γ-CDs. CDs have the capacity to encapsulate hydrophobic molecules within their hydrophobic cavity, based on this nature it is used in the various industries. Leemhuis et al, (2010), Martin Del Valle (2009), Li et al, (2007) and Biwer et al, (2002) reviewed the numerous applications of the CDs in the pharmaceutical, cosmetics, and food and textile industry. CDs are used in the separation of enantiomers to extract toxic chemicals from waste streams (Martin Del Valle, 2009) and in soil bioremediation (Fava and Ciccotosto, 2002). Various other applications of CDs include the suppression of undesirable (bitter) tastes and the extraction of compounds such as cholesterol from foods (Szente and Szejtli, 2004; Szejtli and Szente, 2005)

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