Abstract
The present study investigated the growth of microalgae Chlorella vulgaris in sago wastewater as a substrate for biodiesel production. The optimum conditions, namely pH, temperature, carbon dioxide (CO2), and carbon/nitrogen ratio (C/N), were studied. The central composite design (CCD) was developed using four coded factors for the experimental trials. The maximum algal biomass and lipid production was obtained as 26.50 and 14.10 g/L respectively at an optimum condition of pH of 8, the temperature of 35 °C, CO2 of 1%, and C/N ratio of 80. Different growth kinetic models, namely the Malthus equation, Tessier equation, Lineweaver-Burk plot, and logistic equation, were studied. The Lineweaver-Burk model fits the best modeling equation with a maximum specific growth rate of 0.062 h−1 and a Ks value of 0.86 g/L. The fatty acid profile of Chlorella vulgaris confirmed the presence of palmitic and linolenic acid to a percentage of 45.2 and 32.2%, respectively.
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