Abstract
The production of γ-linolenic acid (GLA) by the fungus Cunninghamella elegans CFR C07 in submerged fermentation was studied. Culture parameters such as carbon source and incubation time were optimized. Four different extraction methods using solvents with acid washed sand, glass beads, lyophilization and Soxhlet extraction were evaluated for improved extraction of lipids from the fungal biomass after fermentation. The GLA production was initially optimized in 250-mL flask and the process was demonstrated in a 3-litre fermentor. The maximum GLA production was 882 mg/L in shake flask culture and 733 mg/L in the fermentor. The study shows that Cunninghamella elegans CFR C07 is a potent organism for the production of GLA under submerged conditions.
Highlights
The present study evaluates the production of good microbial source of γ-linolenic acid (GLA) in submerged fermentation by Cunninghamella elegans CFR C07 isolated at CSIR – Central Food Technological Research Institute (CFTRI), Mysore, India
Glucose was selected as the carbon source in the production medium to increase oil accumulation and biomass yield
Glucose was selected as the carbon source for the production of GLA under submerged conditions
Summary
The aim of this study is to optimize the carbon source, incubation time and extraction methods, and scale up the GLA production using Cunnighamella elegans CFR C07 in submerged fermentation
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