Abstract
An investigation was carried out on alkaline protease production using solid state fermentation from Penicillium godlewskii SBSS 25 isolated from soil samples collected from Southern Kerala, India and its application as a detergent additive. The maximum protease yield (235 U/gds) were achieved with optimized process parameters like pH (9.0), temperature (35°C), moisture content (60%), nitrogen source (0.5% NH4NO3), carbon source (4% glucose) and incubation time (96 h). The enzyme showed stability with a wide range of commercial detergents. These properties make this protease an ideal choice for application in detergent formulations. Key words: Extracellular, alkaline protease, Penicillium godlewskii, optimization, soil, fermentation.
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