Optimization of process parameter for alpha-amylase produced by Bacillus cereus amy3 using one factor at a time (OFAT) and central composite rotatable (CCRD) design based response surface methodology (RSM)

Abstract

Abstract Alpha amylase is a starch hydrolyzing important enzyme which has numerous industrial applications. In the present research an alpha amylase producing Bacillus cereus amy3 was isolated from garden soil of University of North Bengal, India. We have optimized the enzyme production parameters using cheep agro-residues as carbon source through one factor at a time (OFAT) approach. The result obtained from OFAT study indicates that the following medium composition and culture conditions, mosambi peel 1.75% (w/v), medium pH 7, incubation temperature 37 °C, incubation time 48 h, maltose 1% (w/v), Sodium nitrate 0.09% (w/v), tyrosine 0.09% (w/v), inoculum size 3% (v/v) [3 × 107CFU/ml] showed 3122 ± 56 IU/ml of amylase activity which was 3.9 fold higher as compared to unoptimized conditions (800 ± 43 IU/ml). Further optimization was done using central composite rotatable design (CCRD) based response surface methodology (RSM). RSM model was found to have a predicted R2 and Adjusted R2 value of 0.9998 and 0.9997, respectively and can significantly (p