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Abstract
Production of holocellulase by a newly isolated marine Bacillus species via submerged fermentation technique at varying cultivation conditions was investigated. Enzyme production was optimized by altering one variable per time. Moreover effect of lignocellulosic saccharides in inducing cellulase and xylanase production was also investigated. The 16S ribosomal Deoxyribonucleic Acid (16S rDNA) gene sequence analysis exhibited 98% sequence similarity of the isolate with other Bacillus species in the gene bank and was deposited with the accession number KX524510. Optimal xylanase and cellulase production was attained at pH 7, temperature 30°C and agitation speed of 50 and 150 rpm. Furthermore, maximum xylanase and cellulase production were both achieved at 60 h corresponding to the late stationary growth stage, with activity of 16.6 and 0.061 U mL-1 respectively. Xylanase production was maximally induced by beechwood xylan, xylose and arabinose with activities of 13.59, 8.78 and 1.90 (U mL-1) respectively; while cellulase production was induced by carboxymethyl cellulose only and no cellulase activity was detected in the culture supernatant of the other carbon sources tested. Optimization increased cellulase and xylanase yields being 0.006-0.061 and 0.23-16.6 (U mL-1) from unoptimized to optimized respectively. The results of the study suggest the bacterial strain to be a proficient producer of cellulase and xylanase with potentials in biotechnological application.
Highlights
Holocellulase Enzymes, Cellulases (EC 3.2.1.4) and xylanases (EC 3.2.1.8), have increased relevance in several areas of agro-industrial processes including the improvement of nutritional quality of animal feed, quality of dough for various baked products, for cleaning and anti-re-deposition action in detergent industry, cotton softening and denim finishing in textiles industry (Motta et al, 2013)
We report on the optimal conditions for enhanced cellulase and xylanase production by a Bacillus strain, isolated from marine beach sediments in South Africa
Significant difference was observed between the pH ranges (p
Summary
Holocellulase Enzymes, Cellulases (EC 3.2.1.4) and xylanases (EC 3.2.1.8), have increased relevance in several areas of agro-industrial processes including the improvement of nutritional quality of animal feed, quality of dough for various baked products, for cleaning and anti-re-deposition action in detergent industry, cotton softening and denim finishing in textiles industry (Motta et al, 2013) These enzymes could be employed as environment-friendly means of lignocellulosic waste conversion to various bioproducts (de Souza Vandenberghe et al, 2016). Commercial production of cellulases and xylanases is mostly from fungi such as Trichoderma and Aspergillus species, which may have attained maximal yield, having undergone extensive strain improvement over the years (Banerjee et al, 2010; Peterson and Nevalainen, 2012) These commercial enzymes are still limited by the high cost of production, narrow substrate reaction and instability under industrial process dynamics (Motta et al, 2013).
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