Optimization of Preparation Conditions of Crosslinked Potato Starch Microsphere through Orthogonal Experimental Design

Abstract

A lot of studies were done on the base material of drug carriers: liposome, protein microsphere, glutin and high polymer microsphere were all of shortages, but starch has much superiority as the drug carrier. The paper embarked from the analysis the chemical and physical properties of potato starch. According as the characteristic of long chain molecules of potato starch forming microcrystal bundle via inter-molecules hydrogen association under room temperature, which disintegrated in water solution as temperature rising, the better levels of factors, such as gelatinization temperature, gelatinization time, volume of water, pH, according to viscosity of starch gelatinization were select firstly. A W/O reverse emulsion was obtained by dispersing the starch gelatinization into the oil. The effect of emulsifier, evocating agent, crosslinker, crosslinking temperature, and crosslinking time was investigated. An orthogonal experiment of L27(313) was designed to identify the main factors affecting them. In light of range analysis, the comparative importance of factors impacting drug loading was obtained, separately. The effects of parameters were studied using analysis of variance. The results showed that the effective factor to control drug loading were the amount of starch, the volume of water, pH, crosslinking time and the amount of evocating agent, whereas the influences of other factors on drug loading were not significant. In addition, the structure of N, N-Methylenebisacrylamide (MBAA) crosslinked potato starch microsphere (CPSM) was characterized by Fourier transform infrared (FTIR) spectrophotometer and Scanning electron microscope (SEM).