Optimization of Polyethylene Glycol Concentration as an Agglutination Potentiator for Examination of Blood Types of Dry Blood Sample

Abstract

Blood type is classifying blood based on the presence of antigens on the surface of the erythrocyte membrane. In criminal cases, dry blood spots are often used as samples in determining blood types an the examination with the elution absorption method but this method requires a long and complicated time. polyethylene glycol (PEG) has potentiator properties in the formation of agglutination. The purpose was to determine the optimum concentration of PEG of 1%, 2%, 3%, 4%, 5% as an agglutination potentiator. The experimental research method is blood sample dried on wool yarn overnight. the analysis results obtained on the elution absorption method and without PEG as a control is grade agglutination +2, whereas with the addition PEG 5%, 4%, 3%, 2%, 1% obtained grade agglutination + 4, + 4, + 3, + 3, + 3 results. The results statistic showed that there was quality of the results of agglutination grade were different at each additional concentration of PEG. At the addition of 4% PEG gave optimal results. So that the PEG modification method can be used as a agglutination potentiator in the determination of blood groups of dried blood spots.