Optimization of physicochemical parameters influencing the production of prodigiosin from Serratia nematodiphila RL2 and exploring its antibacterial activity.

Abstract

In the present study, role of various physicochemical parameters influencing the production of antimicrobial pigment prodigiosin from Serratia nematodiphila RL2 was determined and optimized. The pigment-producing strain was isolated and based on molecular characterization (16S rRNA sequencing), was identified as S. nematodiphila RL2. The pigment produced by S. nematodiphila RL2 was characterized by thin layer chromatography (Rf 0.94), spectrophotometrically (λmax 535nm) and identified as prodigiosin. Optimization of production parameters of prodigiosin revealed, nutrient broth medium supplemented with lactose and yeast extract at 1% concentration each, have a positive effect on the bacterial growth (10.25-4.6mg/ml DCW) as well as pigment production (0.46-0.6mg/ml). Prodigiosin production (0.64mg/ml) increases optimally after 46-48h of incubation, at 35°C at pH between 6 and 7 with addition of metal ions such as Uranyl acetate. An increase of 65% in prodigiosin production (0.46-0.76mg/ml) was observed after optimizing the various production parameters than unoptimized conditions. Antimicrobial activity of the prodigiosin was also evaluated and found to be effective antimicrobial agent against bacterial pathogens including Listeria sp., Pseudomonas sp., Yersinia sp. and Shigella sp. Present study indicate that S. nematodiphila RL2 is a potent source of pigment prodigiosin which can be further explored for production of prodigiosin.