Abstract
Managing medical procedures for children with problematic disorders is a challenging approach, especially in the case of blood withdrawal for autism spectrum disorder-affected children. Peripheral blood mononuclear cells (PBMC) represent an important cellular model to study immune responses and drug toxicity. The monocytic cells, a fraction of PBMC, are strongly involved in some pathophysiological processes, such as inflammation and immune system changes. Here, we propose a simple, reliable protocol for obtaining peripheral blood-derived mononuclear cells from small volumes of blood samples.
Highlights
Peripheral blood mononuclear cells (PBMC) represent an important cellular model to study immune responses and drug toxicity
Peripheral blood mononuclear cells (PBMC) represent an optimal source of cells for deep studies of several pathophysiological disorders. These cells comprise a heterogenous population of several round nuclei-cell types: monocytes, lymphocytes (B-cells, T-cells) and Natural Killer (NK) cells; differentiated dendritic and macrophagic cells, as well as stem cells, can be present
The specific cellular sub-type composition of PBMC depends on the donor and is based on the donor health status; typically in humans, lymphocytes are most abundant, ranging from 70 to 90%, monocytes are present in 10–20%, and other cells, including dendritic cells, are for only 1–2% [1]
Summary
Peripheral blood mononuclear cells (PBMC) represent an optimal source of cells for deep studies of several pathophysiological disorders. These cells comprise a heterogenous population of several round nuclei-cell types: monocytes, lymphocytes (B-cells, T-cells) and Natural Killer (NK) cells; differentiated dendritic and macrophagic cells, as well as stem cells, can be present. The specific cellular sub-type composition of PBMC depends on the donor and is based on the donor health status; typically in humans, lymphocytes are most abundant, ranging from 70 to 90%, monocytes are present in 10–20%, and other cells, including dendritic cells, are for only 1–2% [1].
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