Abstract
In an attempt to increase the efficiency of soybean transformation by particle bombardment, we examined the effects of bombardment parameters such as gold particle size, target distance, acceleration pressure, amount of DNA per bombardment, and number of bombardments. Transgene delivery to embryogenic tissue grown in suspension culture was evaluated by monitoring the transient expression of a gene for a modified form of jellyfish green fluorescent protein [sGFP(S65T)]. Optimal transient expression of sGFP(S65T) was obtained when the tissue was bombarded twice at an acceleration pressure of 7.6 MPa (1,100 psi) and a distance of 6 cm with gold particles that were 0.6 μm in diameter and coated with 0.8 μg of DNA. Application of these optimized conditions proved effective for the generation of stable transgenic soybean plants. Stable transgene integration in the transformants was confirmed by Southern blot analysis. The average transformation efficiency achieved with the optimized protocol was siginificantly higher than that with the conventional protocol.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
