Abstract
BackgroundPlants with high biomass can be manipulated for their reducing sugar content which ultimately upon fermentation produces ethanol. This concept was used to enhance the production of reducing sugar from cattail (Typha latifolia) by oxalic acid (OAA) pre-treatment followed by enzymatic saccharification. ResultThe optimum condition of total reducing sugar released from OAA pre-treatment was found to be 22.32 mg/ml (OAA—1.2%; substrate concentration (SC)—6%; reaction time (RT)—20 min) using one variable at a time (OVAT). Enzymatic saccharification yielded 45.21 mg/ml of reducing sugar (substrate concentration (SC)—2.4%; enzymatic dosage—50 IU/g; pH 7.0; temp—50 °C) using response surface methodology (RSM). ConclusionWe conclude that Typha can be used as a potential substrate for large-scale biofuel production, employing economical bioprocessing strategies.
Highlights
Plants with high biomass can be manipulated for their reducing sugar content which upon fermentation produces ethanol
We conclude that Typha can be used as a potential substrate for large-scale biofuel production, employing economical bioprocessing strategies
Cellulose matrix is covered with lignin and other polysaccharides which can be converted into sugars by pre-treatment and enzymatic saccharification [22, 31]
Summary
Plants with high biomass can be manipulated for their reducing sugar content which upon fermentation produces ethanol. This concept was used to enhance the production of reducing sugar from cattail (Typha latifolia) by oxalic acid (OAA) pre-treatment followed by enzymatic saccharification. Aquatic weeds have high biomass content and grow uncontrollably in wetlands; they can be used as a potential source for bioethanol production as opposed to agricultural crops that require monetary investment. Cellulose matrix is covered with lignin and other polysaccharides which can be converted into sugars by pre-treatment and enzymatic saccharification [22, 31]. The sugars produced can further be fermented to yield bioethanol, a source of biofuel
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