Optimization of One-Step In Situ Transesterification Method for Accurate Quantification of EPA in Nannochloropsis gaditana

Yuting Tang,Julian Rosenberg,Fei Wang,Yue Zhang,Michael Betenbaugh

doi:10.3390/app6110343

Yuting Tang, Julian Rosenberg + Show 3 more

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https://doi.org/10.3390/app6110343

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Abstract

Microalgae are a valuable source of lipid feedstocks for biodiesel and valuable omega-3 fatty acids. Nannochloropsis gaditana has emerged as a promising producer of eicosapentaenoic acid (EPA) due to its fast growth rate and high EPA content. In the present study, the fatty acid profile of Nannochloropsis gaditana was found to be naturally high in EPA and devoid of docosahexaenoic acid (DHA), thereby providing an opportunity to maximize the efficacy of EPA production. Using an optimized one-step in situ transesterification method (methanol:biomass = 90 mL/g; HCl 5% by vol.; 70 °C; 1.5 h), the maximum fatty acid methyl ester (FAME) yield of Nannochloropsis gaditana cultivated under rich condition was quantified as 10.04% ± 0.08% by weight with EPA-yields as high as 4.02% ± 0.17% based on dry biomass. The total FAME and EPA yields were 1.58- and 1.23-fold higher separately than that obtained using conventional two-step method (solvent system: methanol and chloroform). This one-step in situ method provides a fast and simple method to measure fatty acid methyl ester (FAME) yields and could serve as a promising method to generate eicosapentaenoic acid methyl ester from microalgae.

Highlights

IntroductionAlgae can exhibit high biomass productivities and do not require high quality agricultural condition to grow, and do not directly compete with food crops for arable land [1]
Microalgae represent a promising feedstock for biofuel and nutritional lipid production.Compared to land plants, algae can exhibit high biomass productivities and do not require high quality agricultural condition to grow, and do not directly compete with food crops for arable land [1].In addition, these unicellular organisms can be harvested nearly year-round
The fatty acid methyl ester (FAME) profiles and contents of the in situ procedure were analyzed by gas chromatography (GC) and the reaction conditions of the in situ method were: methanol:biomass = 90 mL/g, HCl 5% by vol, 70 ◦ C, 1.5 h

Summary

Introduction

Algae can exhibit high biomass productivities and do not require high quality agricultural condition to grow, and do not directly compete with food crops for arable land [1]. These unicellular organisms can be harvested nearly year-round. EPA and DHA are commercially available from microalgal sources [7]. Based on this success, efforts have been undertaken to evaluate alternative algal platforms as commercially feasible feedstocks for EPA and DHA at large scales. The estimated production cost for EPA and DHA supplements is

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