Abstract
Liposomes are a delivery system used in pharmaceutical products and cosmetics. Liposomes have many advantages such as increase stability and efficacy, can be targeted to reduce toxicity and increase accumulation at the target site and are biocompatible. Preparation of liposomes can be done by conventional or new methods which are still being developed. Conventional methods often require a long time and organic solvents which may be toxic. Heating (Mozafari method) is one of the new methods developed in the manufacture of liposomes without organic solvents. Mixing temperature can affect the physical properties of liposomes. The particle size has become one of the important physical properties because it affects the absorption of the drug. Sonication is an easy method of choice in reducing the size of liposomes. Optimization of mixing temperature and duration of sonication in liposomes’ preparation using new heating methods and sonication were performed by factorial design with 2 factors and 3-levels to obtain optimal liposome size. Data were analyzed with two-way ANOVA. The results showed that both mixing temperature and sonication duration significantly affect liposome size, but the interaction was not statistically significant. Data analysis also showed that mixing temperature, sonication, and their interaction do not affect the polydispersity index of liposome. Results showed the optimum mixing temperature and sonication duration that can produce liposomes with size below 100 nm is at 60°C for 30 minutes.
Highlights
Liposome is a vesicle which is used as a delivery system for drugs and cosmetics (Barenholz, 2001)
Mixing temperature and duration of sonication in liposome’s preparation using the new combination heating method and sonication needs to be optimized, to obtain an optimum temperature mixing and sonication time, which can be used as a standard condition in making liposomes using the novel method of heating and sonication
Liposomes were prepared by dispersing lecithin in redistilled water with a variety of temperatures using laboratory blender for one minute, the solution was homogenized with Ultraturax for 1 minute and sonicated with variations of mixing temperature and sonication duration
Summary
Liposome is a vesicle which is used as a delivery system for drugs and cosmetics (Barenholz, 2001). Soy lecithin is a phospholipid often used in the formulation of liposomes because it has good stability against variations in pH or the concentration of salt in the formula. Each molecule can move more freely form lipid layers to become liposome This stage is a critical point when formulating liposomes, so mixing temperature needs to be optimized (Knight, 1981). Mixing temperature and duration of sonication in liposome’s preparation using the new combination heating method and sonication needs to be optimized, to obtain an optimum temperature mixing and sonication time, which can be used as a standard condition in making liposomes using the novel method of heating and sonication
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
