Abstract
Methanotrophs-bearing liquid cultivated from the aged refuse has been proved to be effective for improving methane oxidation ability of landfill cover materials, and this is significant for CH4 emission control in landfill site. Optimization of mixed-cultivation conditions of methanotrophs from the aged refuse was studied in this paper. It was demonstrated that the best inoculums mode was using supernatant liquor of the aged refuse and nitrate minimal salt medium (NMS), and the lag phase of the growth was only 37 hours. The optimum growth of methanotrophs was observed at 28~35°C. Paraffin was attested to be useful for mixed methanotrophs cultivation and the highest methane oxidation rate reached 4mL/h with paraffin added.
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