Abstract
The effect of microwave pretreatment on the anaerobic degradation of hyacinth was investigated using response surface methodology (RSM). The components of lignin and the other constituents of hyacinth were altered by microwave pretreatment. Comparison of the near-infrared spectra of hyacinth pretreated by microwave irradiation and water-heating pretreatment revealed that no new compounds were generated during hyacinth pretreatment by microwave irradiation. Atomic force microscopy observations indicated that the physical structures of hyacinth were disrupted by microwave pretreatment. The yield of methane per gram of the microwave-irradiated substrate increased by 38.3% as compared to that of the substrate pretreated via water-heating. A maximum methane yield of 221 mL∙g-sub–1 was obtained under the optimum pretreatment conditions (substrate concentration (PSC) = 20.1 g∙L–1 and pretreatment time (PT) = 14.6 min) using RSM analysis. A maximum methane production rate of 0.76 mL∙h–1∙g-sub–1 was obtained by applying PSC = 9.5 g∙L–1 and PT = 11 min. Interactive item coefficient analysis showed that methane production was dependent on the PSC and PT, separately, whereas the interactive effect of the PSC and PT on methane production was not significant. The same trend was also observed for the methane production rate.
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