Optimization of microwave-assisted solid-phase oligosaccharides synthesis reaction and analysis of components and structure of synthetic product

Abstract

Using phosphoric acid as the catalyst and glucose as substrate, microwave-assisted solid-phase oligosaccharide synthesis was studied. The parameters were optimized by orthogonal test design and statistical analysis method. Under the optimum reaction conditions, namely microwave power output of 1000 W, microwave irradiation time 9 min, adding 30% water to initiate reaction, adding 4% heteropoly acid A as the catalyst, the maximum yield ratio of product was 76.56%. The product was separated and characterized by using SephadexG-50 column, thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC). The component and proportion of synthetic oligosaccharides included disaccharides 20.81%, trisaccharides 15.50%, tetrasaccharide 10.84%, pentasaccharides 7.79%, higher oligosaccharides 21.63%. Results suggest that microwave-assisted solid-phase organic synthesis reaction could be used for the production of oligosaccharides.