OPTIMIZATION OF MICROPROPAGATION ELEMENTS OF INTRODUCED VARIETIES OF GARDEN STRAWBERRY IN AN IN VITRO SYSTEM

Abstract

The purpose of this research is optimization of individual elements of micropropagation of promising introduced varieties of strawberry for use in the production system of healthy planting material. Objects of research are strawberry varieties of foreign selection Asia, Darselect, Siria, Florens, Klery, Honeyoye, Kimberly. The research was carried out in the laboratory of biotechnology of the FGBNU VNIISPK according to generally accepted methods. The optimal sterilizing agent for ensuring maximum sterility, maintaining high viability and rapid stabilization of the introduced strawberry material was 0.1% sulema solution. Elimination of the negative impact of oxidized phenols was achieved by transplanting to a fresh nutrient medium. Introduction into the culture in vitro with the activation of growth processes in strawberry rosettes after winter storage should be considered the most effective for the studied genotypes, which provides rapid stabilization of growth and reproduction, which is the determining factor of intensive production. Varieties of strawberries Asia (56.7%), Siria (52.9%), Florens (45.1%) were characterized by the largest number of explants capable of cloning after two months of cultivation, the least is the variety Klery (19.0%). The ability to form additional axillary micro-shoots was minimal when isolated in the active growth phase and the growth decay phase. The exception was the Asia variety, 33.2% of which explants formed additional microrosettes. The results obtained can be used for the development of systems for the production of healthy planting material of introduced varieties of garden strawberries using biotechnological approaches.