Optimization of metabolite extraction and analytical methods from shrimp intestine for metabolomics profile analysis using LC-HRMS/MS

Abstract

Intestinal microbiota and metabolites play important roles for further improvement of animal production. Metabolomics of shrimp intestine to understand roles and their relationship to the host is hampered by the lack of metabolome profiling method. This study aims to develop extraction and analytical methods to allow accurate metabolic analysis in shrimp intestine. Conditions for extraction and LC-HRMS/MS analysis were optimized. Extraction with ethyl acetate:acetone (15:2 v/v) acidified with 0.5% acetic acid, elution with acetonitrile:water acidified with 0.01% acetic acid for 25min, and mass fragmentation at 15% HCD were the optimal conditions, yielding the highest signal intensity and numbers of putative metabolites. Our method enabled in-depth study for shrimp-microbial interaction at metabolite level.