Optimization of medium for decolorization of Congo red byEnterobactersp. SXCR using response surface methodology

Abstract

AbstractResponse surface methodology based central composite design (CCD) was employed to interpret the decolorization of sulfonated azo dye Congo red by newly isolated bacteria Enterobacter cloacae SXCR. The aim of this work was to evaluate the effect of various components of medium such as KH2PO4, Na2HPO4, NaCl, MgSO4, and trace elements on the dye decolorization. The effects of different sources of carbon and nitrogen sources were also considered for the better performance on the decolorization of Congo red. The influence of these factors on decolorization experiments were performed by newly isolated bacterial strain which was isolated from petroleum contaminates soil samples. However, the effects of salts, carbon, and nitrogen sources were investigated using classical methods while influence of interacting parameters like glucose, peptone, and beef extract on dye removal were examined using CCD. A second-order polynomial model successfully described the effect of independent variables on the dye remov...