Optimization of Medium Constituents for Laccase Production by Trametes Versicolor As 5.48

Abstract

The present investigation provides a report on statistical optimization of medium components to improve Laccase production by Trametes Versicolor As 5.48. Glucose and starch were identified as significant components influencing the biomass and laccase production by T. Versicolor As 5.48 using orthogonal experiment design. The higher laccase production could be obtained when nitrogen source in the medium was at lower level compared with which was for the growth of T. Versicolor As 5.48. In accordance with three significant factors, orthogonal experiment was applied for further optimization to improve the laccase production by T. Versicolor As 5.48. Based on the further optimization results of statistical analyses, the concentrations of medium components for improved laccase production were as follows: Glucose 5.25 g/L, starch 5 g/L, ammonium chloride 67 mM, guaiacol 0.02 mM, potassium dihydrogen phosphate 1 g/L, trace elements 7 mL/L, Vitamin B1 8 mg/L, Vitamin C 1.5 mM, and copper sulfate 0.05 mM. Under the optimized medium, the laccase activity of 17.46 U/mL was attained. From this study, it is evident that orthogonal experiment design can be used to determine the significant variables and the optimum conditions for laccase production, respectively. The optimization of the medium resulted not only in one-fold increase in laccase activity than in the primary optimized medium, but also in a reduction of the constituent number and constituents costs.