Abstract
The interaction between sucrose, yeast extract and initial pH was investigated to optimize critical medium components for mycelium biomass and production of exopolysaccharide (EPS) of Lentinus squarrosulus using Response Surface Methodology (RSM). A central composite design (CCD) was applied and a polynomial regression model with quadratic term was used to analyse the experimental data using analysis of variance (ANOVA). ANOVA analysis showed that the model was very significant (p Lentinus squarrosulus are as follows: sucrose concentration 114.61 g/L, yeast extract 1.62 g/L and initial pH of 5.81; sucrose concentration 115.8 g/L, yeast extract of 3.39 g/L and initial pH of 6.44 respectively.
Highlights
Lentinus squarrosulus is an edible mushroom commonly found in the wild which has recently shown potential medicinal and pharmaceutical properties
The one-factor-at-a-time results indicated that the critical medium components affecting the performance of culture in terms of mycelium biomass and EPS production were the concentration of sucrose, yeast extract and the initial pH of the culture medium
To best of our knowledge, there is no data published on the optimization of medium components for mycelium biomass and EPS production by Lentinus squarrosulus
Summary
Lentinus squarrosulus is an edible mushroom commonly found in the wild which has recently shown potential medicinal and pharmaceutical properties. Due to the long cultivation time of the conventional mushroom cultivation, submerged fermentation is viewed as a promising alternative cultivation method for the efficient growth of mycelium biomass and production of EPS. The one-factor-at-a-time results indicated that the critical medium components affecting the performance of culture in terms of mycelium biomass and EPS production were the concentration of sucrose, yeast extract and the initial pH of the culture medium (unpublished). To best of our knowledge, there is no data published on the optimization of medium components for mycelium biomass and EPS production by Lentinus squarrosulus. In this study, response surface methodology (RSM) was used to determine the optimum medium components for mycelium biomass and EPS production by Lentinus squarrosulus and to better understand the interaction between the medium components (sucrose, yeast extract and initial pH), mycelium biomass and EPS production
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